Photosynthesis studies with tritiated water
نویسندگان
چکیده
منابع مشابه
Glycolic Acid Labeling During Photosynthesis with CO(2) and Tritiated Water.
Chlorella pyrenoidosa were allowed to photosynthesize for short periods of time in the presence of (14)CO(2) and HTO. Analysis of tritium and (14)C labeling of photosynthetic intermediate compounds showed that the T/(14)C ratio of glycolic acid was comparable to that of intermediate compounds of the photosynthetic carbon reduction cycle when photosynthesis was performed in nearly 100% oxygen an...
متن کاملStudies with Tritiated Mithramycin in C3H Mice1
The cytotoxic characteristics of the antibiotic mithramycin were studied by measuring the distribution of tritium-labeled mithramycin in C3H mice. The greatest concentrations of the isotope were in the kidneys and liver. The excretion of mithramycin-3H was rapid, and there was no evidence of metabolism of the carbohydrate moiety to carbon dioxide and water with loss through respiration. Althoug...
متن کاملPhotosynthate transport using tritiated water.
The exchange of HTO with aerial portions of the soybean was studied under a variety of conditions. When fed to a lone leaf or leaflet in a saturated air atmosphere (all other leaves and the growing point having been excised), the HTO profile virtually ceases at a distance of 2 cm from the feeding chamber in the photosynthetic plant, but is greater and more extensive in the unilluminated plant. ...
متن کاملTritium labeling of antisense oligonucleotides by exchange with tritiated water.
We describe a simple, efficient, procedure for labeling oligonucleotides to high specific activity (< 1 x 10(8) cpm/mumol) by hydrogen exchange with tritiated water at the C8 positions of purines in the presence of beta-mercaptoethanol, an effective radical scavenger. Approximately 90% of the starting material is recovered as intact, labeled oligonucleotide. The radiolabeled compounds are stabl...
متن کاملMeasurement of cholesterol biosynthesis in vivo with tritiated water.
The determination of the rate of cholesterogenesis has usually involved either the measurement of the incorporation of a radiolabelled substrate, such as acetate, or the assay of the enzyme /I-hydroxy-p-methylglutaryl-CoA reductase (EC 1.1.1.34), commonly believed to be rate-limiting. However, the use of acetate is of doubtful physiological relevance, and the size and rate of turnover of the fr...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta
سال: 1959
ISSN: 0006-3002
DOI: 10.1016/0006-3002(59)90117-9